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Table 2 SINE200 primer list.

From: Insertion polymorphisms of SINE200 retrotransposons within speciation islands of Anopheles gambiae molecular forms

Locus

SINE200 coordinate

Primer pair (forward/reverse)

Product size (bp)

Annotated genes (in 20 Kb)

S200 X6.1

Chromosome X:

5'-TCGCCTTAGACCTTGCGTTA-3'

479

AGAP001076

 

22951445–22951671

5'-CGCTTCAAGAATTCGAGATAC-3'

 

(CYP4G16 gene)

S200 X6.2

Chromosome X:

5'-TCGGGCCAATATAACACAC-3'

588

AGAP001094

 

24225524–24225731

5'-AGGCGCCATGTACGTAACC-3'

  

S200 2L20A.1

Chromosome 2L:

5'-TGCCCTGTTCAAGATTTCAT-3'

564

none

 

641051–641259

5'-GGTCACTCACGCATACCGTCT-3'

  

S200 2L20A.2

Chromosome 2L:

5'-ACGCCAGACGGTTTCATATC-3'

611

none

 

977287–977497

5'-CCTATCTTTAATTTATATTCGC-3'

  

S200 2L20B.1

Chromosome 2L:

5'-AACCTTACAATACACAAGAAC-3'

495

AGAP004725

 

2796669–2796889

5'-CAGGAAAACGACTACTCGAAC-3'

 

AGAP004726

S200 2L20B.2

Chromosome 2L:

5'-CGCGTTGATTAATAATCCCAC-3'

483

none

 

1191908–1192118

5'-CCAGTCTCTGGACATGCTG-3'

  

S200 2L20B.3

Chromosome 2L:

5'-TTATCTGCGCGTGAGTGG-3'

515

Intron of AGAP004691

 

1276754–1276921

5'-ATACCGCCTAAACGCATG-3'

 

(LIM gene)

S200 2R12D

Chromosome 2R:

5'-AGAATGAATTGTATGGAACAGG-3'

535

AGAP002640

 

24868905–24869106

5'-CTATTAAATGTGTCTCGCTCG-3'

 

(GPR-OR38 gene)

  1. SINE200 locus names, chromosomal locations and coordinates, PCR primers, 'filled' PCR product sizes and annotated genes within 20 Kb including the SINE200 loci investigated are indicated by Ensemble Gene ID, gene names are in brackets.