Var2CSA DBL6-epsilon domain expressed in HEK293 induces limited cross-reactive and blocking antibodies to CSA binding parasites

Table 1 N-Glycosylation sites mutated in HEK293 expressed DBL domains

Domains	Boundaries	N-Glycosylation sites mutated*
DBL1-x	58 – 383	5	N 85I
			N 147Q
			N 356K
			N 362H
			N 380Q
DBL2-x	530 – 863	2	N 692Q
			N 730Q
DBL3-x	1221 – 1548	3	N 1222S
			N 1290Q
			N 1428K
DBL4-ε	1594 – 1888	4	N 1674H
			N 1744Q
			N 1749Q
			N 1844Q
DBL5-ε	2003 – 2270	3	N 2134Q
			N 2210Q
			N 2223Q
DBL6-ε	2322 – 2590	2	N 2442Q
			N 2537Q

*Asparagine residues were changed to glutamine or, in some instances, to amino acids found in other var2csa alleles at this location.

ISSN: 1475-2875