Figure 2

Expression of PfK1 and PfK2. A. Immunoblots of identical synchronized parasite samples (loading 2 × 10⁷ parasites per lane; rich in schizonts and early rings) probed with each of the three anti-PfK1 and two anti-PfK2 antibodies. Similar banding profiles are detected by each anti-PfK1 or anti-PfK2 antibodies. B. Synchronous cultures were sampled over the 48 hr lifecycle and probed by Western Blot (loading ~1 × 10⁷ parasites per lane). PfK1 was detected as a triplet set of bands of >200 kDa using anti-PfK1 F3 antibodies. Expression of PfK1 was detected across the 48 hr lifecycle. PfK2 was detected as two bands of >250 kDa using anti-PfK2 F4 antibodies, with maximal expression detected in late schizonts (ie. samples rich in developing merozoites). Constitutive expression of the ER marker protein GRP(BiP) across the 48 hr lifecycle of P. falciparum was detected at approximately 70 kDa. Stage specific expression of KAHRP, observed as a broad band of reactivity at ~100 kDa, with maximal expression observed in mature trophozoites and schizonts. Minimal expression was detected in samples rich in ring stage parasites. The samples are: rings (R), early trophozoites (ET), late trophozoites (LT) and schizonts (S).