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Table 1 Heterologous expression of 32 potential antimalarial drug targets.

From: Heterologous expression of plasmodial proteins for structural studies and functional annotation

PlasmoDB annotation Product Drug-ability Index (max is 1) MM (Da) pI Heterologous Expression (Ref) Tag Special conditions (e.g. codon adaptation, TM, trunctions) Structure (PDB code)
PF14_0053 Ribonucleotide reductase small subunit 1 40600 5.2 E. coli[212] His and GST - -
PFD0830w Bifunctional dihydrofolate reductase-thymidylate synthase 1 71738 7.2 - E. coli (various) - S. cerevisiae (complemeted) (various) - Cell free systems [207] - - Purposely truncated versions or domains - Codon optimised - S. cerevisiae adapted 1J3I 1J3J 1J3K
PFE0520c Topoisomerase I 1 98110 9.8 - - - -
PFF0160c Dihydroorotate dehydrogenase, mitochondrial precursor 1 65559 9.4 E. coli[213] His N-terminally truncated pRIL tRNA additions 1TV5
PFI1020c Inosine-5'-monophosphate dehydrogenase 1 56151 8.0 - - - -
PF11_0377 Casein kinase 1 0.9 37631 9.7 E. coli[214] His   1lhx
PF14_0192 Glutathione reductase 0.9 56679 8.1 E. coli complementation [215] His Gene complementation 1ONF
PFI1685w cAMP-dependent protein kinase catalytic subunit 0.9 40197 9.1 - - - -
PFI1170c Thioredoxin reductase 0.9 59687 7.8 E. coli[216] His - -
MAL13P1.279 Protein kinase 5 0.9 32997 8.0 E. coli[217] His pRIL tRNA addition 1OB3
PFC0525c Glycogen synthase kinase 3 0.9 51616 5.2 E. coli[218] V5/His-/thioredoxin - -
PFL2250c Rac-beta serine/threonine kinase 0.9 88096 10 E. coli[219] His or GST pRIL tRNA addition -
PF10_0121 Hypoxanthine phosphoribosyltransferase 0.8 70076 4.6 E. coli[220] - - 1CJB
PF10_0165 DNA polymerase delta catalytic subunit 0.8 127072 8.7 E. coli[221] 3-galactosidase - -
PF10_0322 S-adenosylmethionine decarboxylase-ornithine decarboxylase 0.8 168171 6.3 E. coli[222] STREP - -
PF13_0141 L-lactate dehydrogenase 0.8 34108 7.6 E. coli[223, 224] - - 1 TM/1 signal peptide - 2xYT - reducing cell growth temperature to 15°C 1CEQ
PF13_0287 Adenylosuccinate synthetase 0.8 50066 7.7 E. coli[225] - Low-temperature inductions were carried out at 20°C for 12 h 1P9B
PFI1090w S-adenosylmethionine synthetase 0.8 44844 6.7 E. coli[226] His   -
PF14_0076 Plasmepsin 1 precursor 0.8 51461 7.2    1 TM 1PFZ
PF14_0077 Plasmepsin 2 precursor 0.8 51481 5.3 E. coli[227]   - 1 TM - Solubilised and refolded 1SME
PF14_0125 Deoxyhypusine synthase 0.8 57429 6 P. vivax expressed   1 TM -
PF14_0127 N-myristoyltransferase 0.8 47971 8.3 E. coli[228] His - -.
PF07_0029 Heat shock protein 86 0.8 86167 4.7 E. coli[229] His - -
PF14_0164 NADP-specific glutamate dehydrogenase 0.8 52547 7.3 E. coli[230, 231] - - 2bma
PF14_0378 Triose-phosphate isomerase 0.8 27935 6.4 E. coli[232] - - 1lyx
PFD0590c DNA polymerase alpha 0.8 225404 8.4 - - - -
PFE1050w Adenosylhomocysteinase(S-adenosyl-L-homocystein e hydrolase) 0.8 52840 5.7 E. coli[233, 234] - - 1v8b
PFF0730c Enoyl-acyl carrier reductase 0.8 49763 9.5 E. coli[235] MBP pRIL tRNA additions 1nhg
PFI1260c Histone deacetylase 0.8 51376 6.3 Cell free systems [236] No - -
PFI1105w Phosphoglycerate kinase 0.8 45427 8.1 E. coli[237] His - 1lgi
PFD1050w Alpha-tubulin II 0.8 49878 4.6 - - - -
PFD0420c Flap exonuclease 1 0.8 76868 8.1 - - - -
  1. The TDR Targets database (tdrtargets.org) were searched for P. falciparum genes with a druggability evidence index ≥0.7. Hypothetical or putative proteins were excluded.