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Table 1 Experimental L culture trials

From: Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures

Trial name

V (ml)

V RCB (μl)

L (cm)

S (cm2)

V H (μl)

HLD (mm)

p (%)

R 48

P1

16.5

825

0.75

6.8

129.2

0.19

0.4 ± 0.2

0.6 ± 0.8

P2

16.5

825

1

9.0

171.0

0.19

0.6 ± 0.2

1.3 ± 0.8

P3

16.5

825

2

16.0

304.0

0.19

0.7 ± 0.4

2.2 ± 2.1

P4

16.5

825

4

32.0

608.0

0.19

0.9 ± 0.3

3.3 ± 1.6

P5

16.5

825

9

63.6

1208.4

0.19

1.2 ± 0.5

4.1 ± 1.5

  1. Macroscopic geometric characteristic parameters and experimental results of the trials carried out to assess the effect of the distance to the walls of the device on culture parasite development. P5 is the control plate with no internal walls. V refers to the measured total culture volume, V RBC refers to the calculated volume of packed RBCs and V H refers to the calculated volume of the haematocrit layer. L refers to the measured distance between separators and S denotes the calculated base surface. HLD is the calculated depth of the haematocrit layer. p is the average observed parasitaemia and R48 is the calculated growth ratio at 48 hours.