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Table 2 Experimental HLD culture trials

From: Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures

Trial name

V (ml)

V RBC (μl)

D (cm)

S (cm2)

V H (μl)

HLD (mm)

P (%)

R 48

W1

1.0

49.4

3.5

9.62

73.73

0.060. ± 0.015

1.5 ± 1.2

5.2 ± 1.8

W2

1.5

74.1

3.5

9.62

110.60

0.09 ± 0.02

1.6 ± 1.1

5.4 ± 1.9

W3

3.1

155.5

3.5

9.62

194.03

0.19 ± 0.05

1.7 ± 1.3

6 ± 2

W4

5.8

290

3.5

9.62

232.09

0.34 ± 0.08

1.5 ± 1.0

4.9 ± 1.0

W5

10.0

500

3.5

9.62

388.06

0.59 ± 0.15

1.4 ± 1.0

5 ± 2

B1

2.6

130

3.1

7.54

432.84

0.18 ± 0.05

1.8 ± 1.2

6.3 ± 1.2

B2

5.2

260

3.3

8.55

432.84

0.34 ± 0.08

1.7 ± 1.2

6.4 ± 0.9

B3

5.8

290

3.4

9.08

746.27

0.32 ± 0.08

1.7 ± 1.6

7 ± 3

B4

10.1

506.5

3.6

10.1

755.97

0.56 ± 0.14

1.4 ± 0.8

5.1 ± 0.8

B5

10.4

520

3.5

9.62

776.12

0.60 ± 0.15

1.4 ± 1.0

5.1 ± 1.8

B6

20.8

1040

3.9

11.9

1552.24

1.0 ± 0.2

1.1 ± 1.5

3.3 ± 0.7

B7

26.0

1300

4.1

13.2

1940.30

1.1 ± 0.3

1.0 ± 0.4

3.0 ± 0.5

B8

51.5

2074

5

19.6

3841.79

1.5 ± 0.4

0.7 ± 0.2

1.6 ± 0.3

B9

68.6

3432

5

19.6

5122.39

2.0 ± 0.5

0.50 ± 0.19

1.6 ± 0.4

B10

74.1

3705

5

19.6

5529.85

2.2 ± 0.5

0.4 ± 0.2

1.0 ± 0.2

B11

98.8

4940

5

19.6

7373.13

2.9 ± 0.7

0.3 ± 0.16

0.8 ± 0.4

  1. Macroscopic geometric characteristic parameters and experimental results of the trials performed to assess the effect of HLD on the culture development. V refers to the total culture volume, V RBC refers to the calculated volume of packed RBCs, and V H refers to the calculated volume of the haematocrit layer. D refers to the diameter of the haematocrit layer deposit and S denotes the calculated base surface. HLD is the calculated depth of the haematocrit layer, p is the average of the observed parasitaemias and R48 is the calculated growth ratio at 48 h. W refers to cultures in 6-well plastic plates and B to cultures in 5 cm diameter flat-bottom glass bottles. W assays with volumes lower than 1.0 ml have not been presented because they did not develop well, due to the effects of surface tension.