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Figure 1 | Malaria Journal

Figure 1

From: CD36 selection of 3D7 Plasmodium falciparum associated with severe childhood malaria results in reduced VAR4 expression

Figure 1

Serological phenotypes, transcription and adhesion profiles of four 3D7 sub-lines, 3D7 SM , 3D7 UM , 3D7 SM-drift and 3D7 SM-CD36 . Panel (i): Parental 3D7UM and 3D7SM, 3D7SM-drift and 3D7SM-CD36 at the beginning of the experiment and following three (ii) and five (iii) rounds of gelatine flotation or selections for binding to CD36. Panel (A) shows the surface expression of VAR4 (open histograms) detected by flow cytometry using VAR4 anti-CIDR1α mouse (i) and rabbit anti-CIDR1α sera (ii and iii). Reactivity with pre-immunisation sera is shown for comparison (shaded histograms). Histograms are from one out of three replicate assays described in the Figure 2 legend. Panel (B) shows the total percentage of ring-stage transcripts of each var gene group out of total var transcripts (black bars). Grey bars show the percentage transcription of var4 and PFF0010w a group A and B/A gene highly upregulated in the 3D7SM compared to the 3D7UM sub-line. Panel C shows the percentage of iRBC adhering to wild type and CD36-transfected Chinese hamster ovary cells (CHO) compared with the total number of iRBC added to the plate. Bars are means of triplicate wells showing standard deviations and p values. Panel D shows t-tests comparing the recognition (average mean fluorescence intensities, MFI) of the different parasite sub-lines by plasma from 10 children (3–5 years) and 10 adults (12–63 years). The 95% confidence interval, standard deviations, outliers and p values are shown. p values ≤ 0.05 were considered significantly different. The cut-off (reference line) was defined as the mean MFI values obtained using plasma from three Danish donors never exposed to malaria plus two times standard deviation.

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