Figure 3From: CD36 selection of 3D7 Plasmodium falciparum associated with severe childhood malaria results in reduced VAR4 expressionChanges in VAR4 surface expression and transcription of the 3D7 SM subline left to drift (3D7 SM-drift ). Left Y-axis, percentage of infected blood cells positive for VAR4 using flow cytometry and VAR4-specific CIDR1α (closed circle) and DBL5δ (open triangle) rabbit antibodies. Right Y-axis, percentage var4 gene transcription in ring stage (closed square) parasites calculated as: (var4 absolute copy number/∑copy number of all 3D7 var genes) × 100%. Fluorescence of 3D7SM-drift stained with pre-immune serum was used to separate positive cells from cells with background fluorescence.Back to article page