Figure 3

P. falciparum PFE1590w interacts differentially with ApoE isoforms ε2, ε3 and ε4. (A) Yeast diploids containing the indicated plasmids were grown to an OD₆₆₀ = 1.0, serially diluted and spotted onto solid synthetic complete media missing tryptophan and leucine and supplemented with extra adenine (SC-T-L+Ade), and onto plates missing tryptophan, leucine and histidine, and incubated at 30 C for 5–7 days. (B) Proteins were extracted from yeast cells grown in SC-T-L+Ade media to an OD₆₆₀ = 1.0, separated by gel electrophoresis and transferred onto membranes by Western blot. Fusion proteins were detected with antibodies directed towards the Gal4 activation domain (upper panel), and antibodies against actin were used as a loading control (bottom panel).