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Table 1 Variance components of sperm and wing length in A. gambiae and A. stephensi males.

From: Intra-specific variation of sperm length in the malaria vector Anopheles gambiae: males with shorter sperm have higher reproductive success

Experiment 1: A. gambiae sperm length (μm)
Comp df SS MS V (μm2) V (%) F P
male 24 3636431 151518 1012 10.7 4.98 < 0.001
testis 25 760304 30412 365 3.8 2.97 < 0.001
field 229 2345723 10243 206 2.2 1.30 0.002
sperm 2666 21031527 7889 7906 83.3   
total 2944 27773985   9489 100.0   
Experiment 1: A. stephensi sperm length (μm)
Comp df SS MS V (μm2) V (%) F P
male 16 1530523 95658 703 10.9 4.12 0.003
testis 17 394867 23227 275 4.3 3.05 < 0.001
field 178 1355025 7612 251 3.9 1.46 < 0.001
sperm 1854 9682493 5222 5221 80.9   
total 2065 12962908   6451 100.0   
Experiment 2: A. gambiae wing length (mm)
Comp df SS MS V (μm2) V (%) F P
family 31 0.58963 0.01902 3160.6 32.7 3.97 < 0.001
plate 32 0.153325 0.004791 0.0 0.0 0.65 0.911
male 64 0.45960 0.00741 6508.5 67.3   
total 127 1.202555   9669.1 100.0   
Experiment 2: A. gambiae sperm length (μm)
Comp df SS MS V (μm2) V (%) F p
family 31 1655126 53391 62 0.8 1.30 0.231
plate 32 1312648 41020 50 0.6 1.14 0.325
male 64 2308407 36069 577 7.4 4.99 < 0.001
field 512 3700502 7228 10 0.1 1.01 0.407
sperm 5760 41045231 7126 7126 91.1   
total 6399 50021914   7824 100.0   
Experiment 3: A. gambiae wing length (mm)
Comp df SS MS V (μm2) V (%) F p
family 15 0.071471 0.004765 140.8 3.1 1.06 0.416
male 44 0.197288 0.004484 4420.9 96.9   
total 59 0.268759   4561.8 100.0   
Experiment 3: A. gambiae sperm length (μm)
Comp df SS MS V (μm2) V (%) F p
family 15 338142 22543 0 0.0 0.84 0.634
male 45 1212059 26935 354 5.0 3.31 < 0.001
field 244 1985344 8137 150 2.1 1.23 0.012
sperm 2745 18216008 6636 6636 92.9   
total 3049 21751553   7140 100.0   
  1. Experiments 1, 2, and 3: the variance in sperm length (μm) and male wing length (mm) was partitioned into various components (family, tissue culture plate, male, testis, field of view and sperm). For each component the degrees of freedom (df), sum of squares (SS), mean square (MS), the variance (V) with units of μm2 or as a percent (%), the F-statistic (F), and the statistical significance (p) are shown.