Country-wide assessment of the genetic polymorphism in Plasmodium falciparum and Plasmodium vivax antigens detected with rapid diagnostic tests for malaria

Table 1 PCR primers, amplicon sizes and accession number of reference sequences used in the study.

PCR primers named	PCR primers sequence 5' to 3'	Target gene	Amplicons size (bp)	GenBank accession number of reference sequences
				Nucleotide	Protein
PfHRP2-R	TTAATGGCGTAGGCAATGTG	P. falciparum histidine-rich protein II gene, exon 2	905	M13986	AAA51639
PfHRP2-F	TGTGTAGCAAAAATGCAAAAGG
HRP3-R1	TGGTGTAAGTGATGCGTAGT	P. falciparum histidine-rich protein III gene	546	AF202093	AAF14632
HRP3-F2	AAATAAGAGATTATTACACGAAAG
Pf aldolase R1	TTTCCTTGCCATGTGTTCAA	P. falciparum aldolase gene, exon 2	924	J03084	AAA29716
Pf aldolase F1	AGCAGATGTTGCCGAAGAAT
PfLDH-R1	TTTCAGCTATGGCTTCATCAAA	P. falciparum lactate dehydrogenase gene	922	DQ825436	ABH03417
PfLDH-F1	GCACCAAAAGCAAAAATCGT
Pvald PF	CCACTGGATCCGAATATAAA	P. vivax fructose 1,6-bisphosphate aldolase gene	1091	AF247063	AAK43741
Pvald PR	CTTTTCGTAAAGGGATGCT
Pvald NF	CCTCAAACTACCCGCAGAAG		994
Pvald NR	GGAGTTCGCTTCTGCTCTCT
Pvldh PF	GTAGAGGCAGGTGAACACTC	P. vivax L-lactate dehydrogenase gene	1063	DQ060151	AAY59419
Pvldh PR	TCCTTTTAGTCTCCGCAAC
Pvldh NF	AACCCAAAATTGTGCTCGTC		900
Pvldh NR	CGTCGAACTTGGTCTTCTCC

ISSN: 1475-2875