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Table 1 PCR Programme for the genes pfmdr 1 86, pfcrt 76, pfdhps 540, pfdhfr 59, primer and product size

From: Prevalence of pfmdr1, pfcrt, pfdhfr and pfdhps mutations associated with drug resistance, in Luanda, Angola

Gene

Primers

PCR Programme

Product size (bp)

Pfmdr 1 86

754N 754R

Initial step

92°C

3 min

321

  

Denaturation (35×)

92°C

1 min

 
  

Annealing (35×)

51°C

30 s

 
  

Extension (35×)

72°C

1 min

 
  

Final step

72°C

3 min

 

Pfcrt 76 1° nested

76o1F N1R

Initial step

94°C

3 min

528

  

Denaturation (40×)

94°C

45 s

 
  

Annealing (40×)

55°C

45 s

 
  

Extension (40×)

72°C

45 s

 
  

Final step

72°C

3 min

 

Pfcrt 76 2° nested

76 N2F 76 N2R

Initial step

94°C

3 min

271

  

Denaturation (35×)

94°C

45 s

 
  

Annealing (35×)

53°C

45 s

 
  

Extension (35×)

72°C

45 s

 
  

Final step

72°C

3 min

 

Pfdhps 540

540 F 540 R

Initial step

94°C

3 min

439

  

Denaturation (40×)

94°C

1 min

 
  

Annealing (40×)

45°C

1 min

 
  

Extension (40×)

72°C

1 min

 
  

Final step

72°C

3 min

 

Pfdhfr 59

59 F 59 R

Initial step

94°C

3 min

326

  

Denaturation (35×)

94°C

1 min

 
  

Annealing (35×)

55°C

1 min

 
  

Extension (35×)

72°C

1 min

 
  

Final step

72°C

3 min