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Table 1 PCR Programme for the genes pfmdr 1 86, pfcrt 76, pfdhps 540, pfdhfr 59, primer and product size

From: Prevalence of pfmdr1, pfcrt, pfdhfr and pfdhps mutations associated with drug resistance, in Luanda, Angola

Gene Primers PCR Programme Product size (bp)
Pfmdr 1 86 754N 754R Initial step 92°C 3 min 321
   Denaturation (35×) 92°C 1 min  
   Annealing (35×) 51°C 30 s  
   Extension (35×) 72°C 1 min  
   Final step 72°C 3 min  
Pfcrt 76 1° nested 76o1F N1R Initial step 94°C 3 min 528
   Denaturation (40×) 94°C 45 s  
   Annealing (40×) 55°C 45 s  
   Extension (40×) 72°C 45 s  
   Final step 72°C 3 min  
Pfcrt 76 2° nested 76 N2F 76 N2R Initial step 94°C 3 min 271
   Denaturation (35×) 94°C 45 s  
   Annealing (35×) 53°C 45 s  
   Extension (35×) 72°C 45 s  
   Final step 72°C 3 min  
Pfdhps 540 540 F 540 R Initial step 94°C 3 min 439
   Denaturation (40×) 94°C 1 min  
   Annealing (40×) 45°C 1 min  
   Extension (40×) 72°C 1 min  
   Final step 72°C 3 min  
Pfdhfr 59 59 F 59 R Initial step 94°C 3 min 326
   Denaturation (35×) 94°C 1 min  
   Annealing (35×) 55°C 1 min  
   Extension (35×) 72°C 1 min  
   Final step 72°C 3 min