Gene | Primers | PCR Programme | Product size (bp) | ||
---|---|---|---|---|---|
Pfmdr 1 86 | 754N 754R | Initial step | 92°C | 3 min | 321 |
 |  | Denaturation (35×) | 92°C | 1 min |  |
 |  | Annealing (35×) | 51°C | 30 s |  |
 |  | Extension (35×) | 72°C | 1 min |  |
 |  | Final step | 72°C | 3 min |  |
Pfcrt 76 1° nested | 76o1F N1R | Initial step | 94°C | 3 min | 528 |
 |  | Denaturation (40×) | 94°C | 45 s |  |
 |  | Annealing (40×) | 55°C | 45 s |  |
 |  | Extension (40×) | 72°C | 45 s |  |
 |  | Final step | 72°C | 3 min |  |
Pfcrt 76 2° nested | 76 N2F 76 N2R | Initial step | 94°C | 3 min | 271 |
 |  | Denaturation (35×) | 94°C | 45 s |  |
 |  | Annealing (35×) | 53°C | 45 s |  |
 |  | Extension (35×) | 72°C | 45 s |  |
 |  | Final step | 72°C | 3 min |  |
Pfdhps 540 | 540 F 540 R | Initial step | 94°C | 3 min | 439 |
 |  | Denaturation (40×) | 94°C | 1 min |  |
 |  | Annealing (40×) | 45°C | 1 min |  |
 |  | Extension (40×) | 72°C | 1 min |  |
 |  | Final step | 72°C | 3 min |  |
Pfdhfr 59 | 59 F 59 R | Initial step | 94°C | 3 min | 326 |
 |  | Denaturation (35×) | 94°C | 1 min |  |
 |  | Annealing (35×) | 55°C | 1 min |  |
 |  | Extension (35×) | 72°C | 1 min |  |
 |  | Final step | 72°C | 3 min |  |