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Table 2 Fragments length, clones and enzymes used for digestion of the codons 86 Pfmdr 1, 76 Pfcrt, 540 Pfdhps and 59 Pfdhfr.

From: Prevalence of pfmdr1, pfcrt, pfdhfr and pfdhps mutations associated with drug resistance, in Luanda, Angola

Gene

Enzyme

clone

Codon

Fragments length

Pfmdr1 86

Apo I

3D7

AAT (Asn)

249 + 72 bp

  

Dd2

TAT (Tyr)

321 bp

Pfcrt 76

Apo I

3D7

AAA (Lys)

137 + 124 + 10 bp

  

K1

ACA (Thr)

261 + 10 bp

Pfdhps 540

BseG I

N3

GAA (Glu)

354 + 85 bp

  

T9/94

AAA (Lys)

439 bp

Pfdhfr 59

Pdm I

K1

CGT (Arg)

354 + 85 bp

  

HB3

TGT (Cys)

439 bp