Skip to main content

Table 2 Fragments length, clones and enzymes used for digestion of the codons 86 Pfmdr 1, 76 Pfcrt, 540 Pfdhps and 59 Pfdhfr.

From: Prevalence of pfmdr1, pfcrt, pfdhfr and pfdhps mutations associated with drug resistance, in Luanda, Angola

Gene Enzyme clone Codon Fragments length
Pfmdr1 86 Apo I 3D7 AAT (Asn) 249 + 72 bp
   Dd2 TAT (Tyr) 321 bp
Pfcrt 76 Apo I 3D7 AAA (Lys) 137 + 124 + 10 bp
   K1 ACA (Thr) 261 + 10 bp
Pfdhps 540 BseG I N3 GAA (Glu) 354 + 85 bp
   T9/94 AAA (Lys) 439 bp
Pfdhfr 59 Pdm I K1 CGT (Arg) 354 + 85 bp
   HB3 TGT (Cys) 439 bp