Plasmodium vivax dhfr and dhps mutations in isolates from Madagascar and therapeutic response to sulphadoxine-pyrimethamine

Table 4 Paired analysis of pvcsp, pvmsp3 and microsatellite markers sequences used for differentiating recrudescences from reinfections for the four patients with a reoccurrence of parasitemia (Tsiroanomandidy, Madagascar, 2006).

		Allele sizes of microsatellites markers (bp)						Genotypes in
Patient	Day	6.34	L34	2.21	8.332	14.185	L40	*pvcsp gene*	*pvmsp3 gene*	Final classification
TDD062079	0	221/225	108	196	217	89/91	97	VK210	A	Recrudescence
	1	221/225	108	196	217	91	97	VK210	A
	14	225	108	196	217	91	97	VK210	A
TDD06viv12	0	219	108/110	196	222	na	97	VK210	na	Recrudescence
	1	219	108/110	196	222	na	97	VK210	na
	28	219	110	196	222	na	97	VK210	na
TDD06viv15	0	225	110	196	na	98	97	VK210	na	Reinfection or Relapse
	1	230	104	205	na	93	97	VK210	na
	21	225	108	196	na	91	97	VK210	na
TDD06viv20	0	230	108	198	na	91	97	VK210	A	Recrudescence
	1	230	108	198	na	91	97	VK210	A
	14	230	108	198	na	91	97	VK210	A

na: not available
Two infections (TDD062079 and TDD06viv12) were polyclonal as indicated by microsatellite markers analysis.
pvcsp sequences were classified as VK210 or VK247 types as described [17]; after DNA sequences alignment, no SNP was observed between day 0, 1 and the day of reoccurent parasitemia.
pvmsp3 sequences were classified into types A (1900 bp), B (1300 bp) or C (1100 bp) as described [40]; after DNA sequences alignment, one SNP was observed between TDD062079 at day 0 and the sequences obtained from both days 1 and 14. No differences were observed between TDD06viv20 sequences.

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