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Table 4 Paired analysis of pvcsp, pvmsp3 and microsatellite markers sequences used for differentiating recrudescences from reinfections for the four patients with a reoccurrence of parasitemia (Tsiroanomandidy, Madagascar, 2006).

From: Plasmodium vivax dhfr and dhps mutations in isolates from Madagascar and therapeutic response to sulphadoxine-pyrimethamine

   Allele sizes of microsatellites markers (bp) Genotypes in  
Patient Day 6.34 L34 2.21 8.332 14.185 L40 pvcsp gene pvmsp3 gene Final classification
TDD062079 0 221/225 108 196 217 89/91 97 VK210 A Recrudescence
  1 221/225 108 196 217 91 97 VK210 A  
  14 225 108 196 217 91 97 VK210 A  
TDD06viv12 0 219 108/110 196 222 na 97 VK210 na Recrudescence
  1 219 108/110 196 222 na 97 VK210 na  
  28 219 110 196 222 na 97 VK210 na  
TDD06viv15 0 225 110 196 na 98 97 VK210 na Reinfection or Relapse
  1 230 104 205 na 93 97 VK210 na  
  21 225 108 196 na 91 97 VK210 na  
TDD06viv20 0 230 108 198 na 91 97 VK210 A Recrudescence
  1 230 108 198 na 91 97 VK210 A  
  14 230 108 198 na 91 97 VK210 A  
  1. na: not available
  2. Two infections (TDD062079 and TDD06viv12) were polyclonal as indicated by microsatellite markers analysis.
  3. pvcsp sequences were classified as VK210 or VK247 types as described [17]; after DNA sequences alignment, no SNP was observed between day 0, 1 and the day of reoccurent parasitemia.
  4. pvmsp3 sequences were classified into types A (1900 bp), B (1300 bp) or C (1100 bp) as described [40]; after DNA sequences alignment, one SNP was observed between TDD062079 at day 0 and the sequences obtained from both days 1 and 14. No differences were observed between TDD06viv20 sequences.
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Malaria Journal

ISSN: 1475-2875

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