Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species

Table 2 Enrichment of gametocytes obtained with P. falciparum from infected blood from patients and in vitro cultures

	Initial^a	Eluent^b	Final count^c	Enrichment rate^d
Patients blood^e	21	2	1120	53
	6	1	514	85
	19 (Fig. C)	1	819 (Fig. D)	43
In vitro culture	0.8 ± 0.03%^f	< 0.01	91%	114
Stage II–III	0.9 ± 0.1%	< 0.01	88%	98
In vitro culture Stage IV–V	0.4 ± 0.02%	< 0.01	95%	237
	0.8 ± 0.1%	< 0.01	92%	115
	0.7% ± 0.1%	< 0.01	94% (Fig. 1-E)	134

a: calibrated thick films (5 μL), gametocyte count
b: calibrated thick films (5 μL), gametocyte count after elution from the columns
c: Calibrated thick films (5 μL), gametocyte count on the retained blood
d: calculated as ratio of final/initial counts
e: estimated on three patients with presence of gametocytes
f: estimated on thin blood smears (at least 10,000 red blood cells were counted).

ISSN: 1475-2875