Skip to main content
Figure 2 | Malaria Journal

Figure 2

From: Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum

Figure 2

Output file of the MCA assay with plasmid DNA. The figure shows the melt curve analysis of wild type clone PS-Mali in triplicate (red curves) resulted in an average Tm of 55.7°C. In green the melt curve profile (in triplicate) of mutant clone PS-Peru is shown with a Tm of of 51.9°C. The change in amount of fluorescence for each probe-template hybrid was plotted against the temperature and its negative derivative appeared as a positive peak. The grey lines represent the negative controles.

Back to article page

Malaria Journal

ISSN: 1475-2875

Contact us