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Figure 3 | Malaria Journal

Figure 3

From: Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum

Figure 3

Identification of a polyclonal infection. Blood samples from two patients with malaria that have a biclonal infections. The MCA curves of these samples with two peaks represented in blue and purple are shown. The sample represented in purple contains according to the intergrated software a 45% to 55% ratio of mutant and wild type infection respectively. The sample represented in blue shows the opposite pattern and contains 55% to 45% mutant and wild type infection respectively. This can also be seen in the figure by the difference in peak hight. The red curves are the positive controls for wild type strains and the green for mutant strains. The blue horizontal line represents the threshold for background fluorescence, and the curve entirely below shows the results for the negative control (gray).

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