Figure 1
From: Role of Plasmodium falciparum thrombospondin-related anonymous protein in host-cell interactions
(a). Gel-filtration analysis of recombinant TRAP ECD and subdomains. Ni-affinity-purified recombinant PfTRAP A domain, A+RII domain, A+RII+RGD domain and ECD expressed in HEK 293 cell migrate on a gel-filtration columns (GPC) as monomers. (b). SDS-PAGE of TRAP subdomains under reducing conditions. The mammalian cell-expressed and purified PfTRAP A domain (lane 1), A+RII domain (lane 2), A+RII+RGD domain (lane 3) and ECD (lane 4) migrate at a molecular mass of approx. 33, 36, 38 and 70 kDa under reducing conditions.