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Figure 3 | Malaria Journal

Figure 3

From: Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants

Figure 3

Sensitivity, inter- and intra-assay variabilities of P. falciparum DNA quantification. (A) Results obtained from four independent tests in duplicate by real-time PCR assay using FRET. (B) Linearity of FRET assay PCR is shown using serially diluted P. falciparum DNA (5000 to 0.25 parasites per reaction, one representative experiment). Amplification efficiency (AE) is calculated based on the slope of the standard curves using the formula: E = 101/-s-1, where E(100) is the % efficiency and s is the slope of the standard cure. (C) Amplification of serially diluted P. falciparum DNA (5000 to 5 parasites per PCR reaction) by conventional PCR assay followed by gel electrophoresis and ethidium bromide staining.

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