Skip to main content

Table 2 Relative standard deviations (RSDs) and recoveries for NO2- and NO3- from mosquito midgut spiked with 1–100 μM KNO2/KNO3 (mean ± SD, n= 6)

From: Determination of nitric oxide metabolites, nitrate and nitrite, in Anopheles culicifacies mosquito midgut and haemolymph by anion exchange high-performance liquid chromatography: plausible mechanism of refractoriness

Concentration (μM)

NO2-

NO3-

Intra-Assay RSD (%)

Inter-Assay RSD (%)

Recovery (%)

Intra-Assay RSD (%)

Inter-Assay RSD (%)

Recovery (%)

0*

8.3

8.9

-

4.2

5.6

-

1

8.8

9.3

94.4 ± 4.4

8.7

9.9

98.9 ± 2.2

2.5

7.1

9.8

98.2+6.6

7.2

8.5

98.5 ± 2.4

5

6.7

9.9

95.2 ± 2.9

3.9

5.9

97.1 ± 1.8

10

5.1

9.2

98.5 ± 3.1

3.1

2.9

99 ± 5.4

25

4.5

5.3

99.8 ± 1.9

2.2

3.0

100 ± 5.8

50

5.5

4.9

100.5 ± 3.2

2.9

2.6

94.5 ± 6.2

100

3.9

4.7

97.3 ± 2.9

1.2

4.1

94.7 ± 5.9

  1. * Unspiked midgut samples, i.e. midgut samples to which exogenous NO2- and NO3- has not been added