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Table 2 Primers for amplification and sequencing of Pvdhfr-ts and flanking, using PCR product B (amplifies the region from -3258 bp upstream to -420 bp upstream of P. vivax dhfr-ts). All sequences are written 5' to 3'

From: Multiple origins of resistance-conferring mutations in Plasmodium vivax dihydrofolate reductase

Primer name Use Sequence
S1238 PCR 1st nested ATC AAG GAA GGC AGA CTC CA
S1239 PCR 1st nested AGC GTA CTG CCG TCG AAA TA
S1259 PCR 2nd nested, sequencing GCC TGG TTA CTT TTG GTG GA
S1240 PCR 2nd nested, sequencing AAA AAC TGA GGC CAC ATT CG
S1241 sequencing ACT TCT CTC CTG GGC AGA CTT
S1242 sequencing GAG AGT TGG TAA TGC GGG G
S1243 sequencing CAT GGC TGG GGA AGG CTC
S1244 sequencing CCC TTA ACC CGC ATG CAC
S1245 sequencing CTC CCC CCA TGG GAC AAA AA
S1246 sequencing TTT GAT TTG ATT TGA TTT GAT TTG A
S1247 sequencing ATG CCA CAG GGA AGT TAC AG
S1248 sequencing CAT TTT TCA CAT TTT GGA AA
S1249 sequencing CCT CGC GCG GGG GGG AAA
S1250 sequencing TGC TGC AAT GCA AGT GGG T