Table 2 Primers for amplification and sequencing of Pvdhfr-ts and flanking, using PCR product B (amplifies the region from -3258 bp upstream to -420 bp upstream of P. vivax dhfr-ts). All sequences are written 5' to 3'
From: Multiple origins of resistance-conferring mutations in Plasmodium vivax dihydrofolate reductase
Primer name | Use | Sequence |
|---|---|---|
S1238 | PCR 1st nested | ATC AAG GAA GGC AGA CTC CA |
S1239 | PCR 1st nested | AGC GTA CTG CCG TCG AAA TA |
S1259 | PCR 2nd nested, sequencing | GCC TGG TTA CTT TTG GTG GA |
S1240 | PCR 2nd nested, sequencing | AAA AAC TGA GGC CAC ATT CG |
S1241 | sequencing | ACT TCT CTC CTG GGC AGA CTT |
S1242 | sequencing | GAG AGT TGG TAA TGC GGG G |
S1243 | sequencing | CAT GGC TGG GGA AGG CTC |
S1244 | sequencing | CCC TTA ACC CGC ATG CAC |
S1245 | sequencing | CTC CCC CCA TGG GAC AAA AA |
S1246 | sequencing | TTT GAT TTG ATT TGA TTT GAT TTG A |
S1247 | sequencing | ATG CCA CAG GGA AGT TAC AG |
S1248 | sequencing | CAT TTT TCA CAT TTT GGA AA |
S1249 | sequencing | CCT CGC GCG GGG GGG AAA |
S1250 | sequencing | TGC TGC AAT GCA AGT GGG T |