Identification of phosphorylated proteins in erythrocytes infected by the human malaria parasite Plasmodium falciparum

Wu, Yang; Nelson, Morag M; Quaile, Andrew; Xia, Dong; Wastling, Jonathan M; Craig, Alister

doi:10.1186/1475-2875-8-105

Table 2 The proteins possess significant ion score for phosphorylated peptides with defined phosphorylation sites

From: Identification of phosphorylated proteins in erythrocytes infected by the human malaria parasite Plasmodium falciparum

Plasmodium falciparum proteins from ItG-pRBCs:
No*	Accession no	Protein name	Phosphorylated peptide sequences	MW*	Site*	Score*
PF1	PFB0100c	knob-associated His-rich protein	GASTTAGSTTGATTGANAVQSK	69791	T5, S8	103
PF2	PFE0870w	transcriptional regulator	FAYKSDEDDEGYNK	133456	S5	67
PF3	PF08_0137	hypothetical protein PF08_0137	KGSLGFDSFK	147208	S3	46
PF4	PF10_0159	Glycophorin-binding protein (GBP-130)	SVVTEEQKVESDSEK	90077	S11	37
PF6	MAL7P1.38	regulator of chromosome condensation protein	INEQSEGSNLPSEQNK	79931	S5	31
PF7	PFL0050c	hypothetical protein PFL0050c	AVPQNSGSNFDEFLDVK	77525	S8	32
PF5	PFI0265c	RhopH3	GLEFYKSSLK	104856	S7	33
PF8	PFD1165w	protein kinase, conserved	INDPYDYLKSITNQEER	75365	S10	61
PF9	PFE1485w	hypothetical protein	ETNESIYIK	225530	T2, Y7	45
PF10	PF14_0648	hypothetical protein	CDKEIYNLHIK	238305	Y6	36
PF11	PFE1600w	hypothetical protein	NYSTHENTYPTLK	62720	Y9	49
PF12	PF14_0434	hypothetical protein	FSVFDSDDNSEEEIENK	41909	S6, S10	42
			IIEVHDNIPSPIIK		S10	75
			DLNESPKNEPDIVYEEK		S5	59
PF13	PF13_0214	elongation factor 1-gamma	DDNNNNNNNDADNQHADLLSDDLAEK	50491	S20	48
PF14	MAL8P1.95	hypothetical protein	NLENNEDDETNVGR	37933	T10	44
PF15	PF07_0008	hypothetical protein	HYSLGGQPSSSGR	27713	S9	76
			NYSLGNLSTGTTSQGSTSSR		T11	76
			YNTSNLASSSTTESSVSGLNTNEAHV		S10	78
			NYSLGNLSTGTTSQGSTSSR		Y2 S16	58
			YNTSNLASSSTTESSVSGLNTNEAHV		T12	92
PF16	PFL1005c	chromodomain protein	NESPQWVEETNIR	30999	S3	55
			TGSDEEFEIGDILEIK		S3	98
PF17	MAL7P1.174	hypothetical protein	DGNDSSSEELDNTNVPTSKPK	37984	S5	63
PF18	PF13_0275	hypothetical protein	YGSGSHDHEEEVVEA	32877	S3	35
			SSSNSHVESNTFQNK		S3	47
PF19	XP_001351065.1	RESA-like protein	LNTIGNLFEGEK	36224	T3	54
PF20	Q8IKH8_PLAF7	ribosomal protein S3	TGLTSILPDNISVLEPK	24823	S12	46
PF21	PFD0375w	hypothetical protein	EYMNNILYDQNK	142869	Y2	36
Human proteins in ItG-pRBCs:
Hu1	SPTB1	spectrin beta isoform b	KEELGELFAQVPSMGEEGGDADLSIEK	246894	S13	89
Hu2	M28880	Ankyrin	LGYISVTDVLK	207138	S5	62
Hu3	BAD92655	Ankyrin 1 isoform 4 variant	ITHSPTVSQVTER	208408	S4	55
Hu4	CAA41149	Erythrocyte alpha adducin	SPGSPVGEGTGSPPK	81377	S4	47
			AAVVTSPPPTTAPHK		S6	55
Hu5	AAH56881	ADD2 protein	TESVTSGPMSPEGSPSKSPSK	78576	S18	56
			SAGPQSQLLASVIAEKSR		S17	72
Hu6	AAH04261	Proline rich 7 protein	KIVTPFLSR	28986	T4	45
Hu7	MMHUE4	Erythrocyte membrane protein band 4.1	SLDGAAAVDSADR	95750	S1	65
Hu8	C3HU	Chain A, Complement Component C3	SGIPIVTSPYQIHFTKTPK	188585	T17	21
Hu9	Q05764	Beta-adducin	SPGSPVGEGTGSPPK	81129	S4	89
Hu10	CAH93400	Erythrocyte membrane protein band 4.9	SSSLPAYGR	45600	S3	38
Hu11	AAC50223	Dematin 52 kDa subunit	RGAEEEEEEEDDDSGEEMK	45726	S14	50
Hu12	NP_001284	Chloride ion current inducer protein I (Cln)	EPVADEEEEDSDDDVEPITEFR	26084	S11	55
			FEEESKEPVADEEEEDSDDDVEPITEFR		S17	60
Hu13	CAA40340	Glycophorin A	SPSDVKPLPSPDTDVPLSSVEIENPETSDQ	14784	S19, T27	43
Hu14	GFHUC	Glycophorin C	GTEFAESADAALQGDPALQDAGDSSR		S25	80
Human proteins from normal RBCs:
RBC1	BAD92652	Spectrin, beta, erythrocytic	DASVAEAWLIAQEPYLASGDFGHTVDSVEK	268238	S27	62
			LSSSWESLQPEPSHPY		S4	27
RBC2	NP_000338	Spectrin	TSPVSLWSR	246468	S2	26
RBC3	NP_976217	Erythrocyte membrane protein band 4.1	VSLLDDTVYECVVEK	71955	Y9	58
			SLDGAAAVDSADR		S1	81
RBC4	AAL15446	Erythrocyte membrane protein 4.1N	TETMTVSSLAIRK	97405	S7 S8	23
RBC5	NP_001969	Erythrocyte membrane protein band 4.9	RGAEEEEEEEDDDSGEEMK	45514	S14	70
RBC6	CAA34611	Alt. ankyrin (variant 2.2)	ITHSPTVSQVTER	189011	S4	64
RBC7	NP_054909	Adducin 1 (alpha) isoform c	AAVVTSPPPTTAPHK	69985	S6	58
			SPGSPVGEGTGSPPK		S4	74
RBC8	AAH56881	ADD2 protein	TESVTSGPMSPEGSPSKSPSK	78576	S18	54
RBC9	NP_002427	Palmitoylated membrane protein 1	TAELSPFIVFIAPTDQGTQTEALQQLQK	52296	T20	53
RBC10	2GU8_A	Chain A, Discovery Of 2-Pyrimidyl-5-Amidothiophenes As Novel And Potent Inhibitors For Akt	TWDLCGTPEYLAPEIILSK	39232	T1	43
RBC12	1BUW_B	Chain B, Crystal Structure Of S-Nitroso-Nitrosyl Hemoglobin A	GTFATLSELHADK	15875	S7	31
RBC13	1DPF_A	Chain A, Crystal Structure Of A Mg-Free Form Of Rhoa Complexed With Gdp	DQFPAVYVPTVFENYVADIEVDGK	20169	Y7	23

No*: PF = Plasmodium protein; Hu = erythrocyte protein; MW* = protein molecular weight; site* = phosphorylated site identified by MASCOT; Score* = All phosphorylated peptide ion scores reached a significant level

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ISSN: 1475-2875

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