Co-ordinated stage-dependent enhancement of Plasmodium falciparum antioxidant enzymes and heat shock protein expression in parasites growing in oxidatively stressed or G6PD-deficient red blood cells

Table 1 Primer pairs of amplicons analysed by qRT-PCR

Gene Name	Gene ID (Entrez Nucleotide NCBI)	Gene ID (Plasmo DB)	(5' → 3') Primer Sequences		Prod. Tm	Ampl. size	Effic.
			Forward	Reverse	°C	(bp)	(%)
18S rRNA	M19172	MAL1_18s	CAAGGAAGTTTAAGGCAACAACAG	CATATCTTTCAATCGGTAGGAGCG	86	313	100.9
GAPDH	AF030440	PF14_0598	TGGAATTGTTGAAGGTTTAATG	CTGGAATAATGTTGGATAATGC	85.5	128	98.8
GPx	Z68200	PFL0580w	TCGATGCATGATGAAAATGGAACG	AATCTAACGGGTTTGTTTTGGGTG	82	118	100.3
GR	AF027825	PF14_0192	GTTTAGGTGGAACGTGTGTCAAC	GGTGTCAAATCCGTAATGCCTGG	83.5	110	99.3
Grx	AF276083	PFC0271c	CCCATATTGTATTAAGGCAATT	TTCTCAATGTTTTCTACATGC	79	78	100.3
GST	AY014840	PF14_0187	GCTTGGAGATAATATAGTGTTATA	CTATTCCAAGGTAGGCAAAA	78.5	86	100.2
1-Cys-Prx	AF294425	PF08_0131	GGGCTATTTTATTTAGTCACCCAC	TTGATCGTGAGATTCTTTGGAGTT	83	146	96.6
2-Cys-Prx	AB037568	PF14_0368	TGGTAGATCAGTTGAGGAAGTCTT	CACTAACACCTTCTTCTGATGGTT	85.5	131	97.4
TR	X87095	PFI1170c	ATGGTGCATGTGGATATTCAGAAG	TGTCGATGAACAGCAGATATTTCC	80	177	99.8
SOD-1	Z49819	PF08_0071	TTGTGGTGGTGAGCCTCATGG	CCCCAACCGGAACCAAAATGAC	82	84	100.1
SOD-2	AY586514	PFF1130c	TTTAACCACAATTTCTTTTGGC	CACTTCCAAAATGACCTGATG	78.7	151	98.7
Hsp60¹	U38963	PF10_0153	CCAGTCATCCTTATTAGTTATAGC	CTATATCATGAATCAAGGCTTTTC	82.5	149	99.5
Hsp70–2²	M18836	PFI0875w	ATGCCAACCAATCATTGTTAAATT	ATTCGTCACTATCTACATCTTCGT	84	89	100.5
Hsp70–3³	AB050740	PF11_0351	TTAGTTGGTGGTATGACTAGAATG	TTAATACACCTCCTTGTATAGCAG	83.5	133	100
Hsp75⁴	M28261	PF08_0054	AATTTCCCAGGAGGTATGCCC	TTCTTCAACTGTTGGTCCACTTC	90	75	97.9
Hsp90⁵	X13014	PF07_0029	CATGACTAGCTATATGTTATCCAA	AAGAGGTATCAAATAATAACCAGA	81.5	143	100.3

¹PlasmoDB nomenclature (PDB): hsp60; ² PDB: Heat shock protein (72 kDa); ³PDB: Heat shock protein hsp 70 kDa homologue (73 kDa); ⁴PDB: heat shock 70 kDa protein (75 kDa); ⁵PDB: heat shock protein 86.

ISSN: 1475-2875