Figure 1

Agarose gel showing PCR products obtained from laboratory line and Kilifi isolate parasite genomic DNA. Gel electrophoresis of nested stevor PCR products from genomic DNA extracted from 5 representative P. falciparum laboratory lines: A4; FcB1; C10; T9/96; and 3D7 (A) and 10 representative Kilifi parasite isolates (B). 5 μl of each product was loaded onto a 2.5% agarose gel. Results show two amplicons in the expected size ranges, products carried over in the nested PCR-step from the external PCR (approximately 600 bp) with primers smf1 and smr1, and internal PCR products (approximately 300 bp) from primers RepF1/2 and RepR. 100 bp marker (Fermentas) is shown. dH₂O = negative control containing sterile water instead of template DNA.