VAR2CSA construct boundaries. VAR2CSA protein schematic showing original domain boundaries in black and revised domain boundaries in grey. The start and stop positions for each construct are shown; IT4-var2csa allele in black, 3D7-var2csa in green, 7G8-var2csa in blue, Dd2-var2csa in red and Pc49-var2csa in orange. Thick bars indicates the recombinant protein was secreted by P. pastoris, thin bars means it was not. The presence of predicted C11 and C12 cysteine residues at the C-terminus of constructs is indicated by two asterisks.