Figure 4From: Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastorisVAR2CSA recombinant proteins secreted by P. pastoris. 1 μg of purified P. pastoris recombinant proteins were analysed in 4–20% SDS-PAGE gels under non-reduced (A, B) or reduced (C) conditions. Proteins were stained with Gel Code Blue Reagent in (A) and (C) or were detected by Western blot with an anti-His Tag antibody (B).Back to article page