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Table 2 Effect of methanol concentration and induction time on recombinant protein yield

From: Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris

  

Methanol concentration and induction time

  

0.50%

1%

Construct

 

48 h

120 h

120 h

var2csa 7G8 DBL5

Q2000-T2318

1.2 mg/L

0.9 mg/L

Nt

var2csa IT DBL2-ID2

D517-G1229

0

0

0

var2csa IT DBL3

L1221-E1541

0.1 mg/L

0.375 mg/L

1 mg/L

var2csa 3D7 DBL5

N1888-T2291

0.21 mg/L

0.46 mg/L

Nt

var2csa 7G8 DBL4-DBL5

G1578-2318

0

0.1 mg/L

Nt

var22 IT DBL3

G1180-N1488

0

nt

0.75 mg/L

var18 IT DBL3

N1247-D1545

0

nt

0.125 mg/L

  1. P. pastoris clones were grown overnight at 0.9 L to build biomass and protein was induced at 0.3 L scale at different durations and methanol concentrations. nt, not tested.