Skip to main content
Figure 1 | Malaria Journal

Figure 1

From: Temporal stability of naturally acquired immunity to Merozoite Surface Protein-1 in Kenyan Adults

Figure 1

Temporal stability of five immunologic assays at various time intervals. X axis: The first group of columns represents assays performed at four different 3-week intervals (Aug. 24 to Sept. 15, Sept. 15 to Oct 6, Apr. 6 to May 5, May 5 to May24). The second group of columns represents assays performed at two different 6-week intervals (Aug. 24 to Oct. 6, Apr. 6 to May 24). The single columns at far right represent assays performed at a single 6-month (Oct. 6 to Apr. 6) and 9-month interval (Aug. 24 to May 24). Horizontal dotted lines across 3-week or 6-week columns indicate average kappa scores. Sample number comparison represented by each column is 16 unless otherwise specified. Y axis: Kappa score: Scores of 0.81–1.0, 0.61–0.80, 0.41–0.60, 0.21–40, and <0.20 indicate, respectively, almost perfect agreement and high stability, substantial stability, moderate stability, fair stability, and slight to no stability. A: 3D7 MSP-142 stimulated INF-γ production measured by ELISPOT. Kappa scores indicate moderate temporal stability over 3-week intervals (0.41) with fair stability (0.22) at 6-week intervals. Sample number for each column from left to right is 15, 10, 11, 12, 14, 16, and 16. B: 3D7 MSP-142 IgG measured by ELISA. Kappa scores indicate high (0.86) and substantial temporal stability (0.72) over 3-week and 6-week intervals. At 6-month and 9-month intervals, stability was fair and moderate. C: FVO MSP-142 IgG measured by ELISA. Kappa scores indicate high to substantial temporal stability for all time intervals tested. The average kappa score for 3-week and 6-week intervals is 0.91 and 0.82. D: MSP-119 IIA. Kappa score at 3-week interval (0.31) indicates fair temporal stability that disappears at longer time intervals. E. Growth Inhibitory Assay (GIA). Kappa scores at 3-week (0.37), 6-week (0.51), and 6-month interval indicate fair to moderate stability. The kappa score at the 9-month interval (0.20) indicated the lowest degree of temporal stability.

Back to article page