Implementation of a novel PCR based method for detecting malaria parasites from naturally infected mosquitoes in Papua New Guinea

Table 1 Results of the comparisons between CS-ELISA (ELISA) and Cytb-PCR (PCR) using artificially infected mosquitoes

	Pv-210		Pv-247		Pf
	ELISA	PCR	ELISA	PCR	ELISA	PCR
Unfed mosquitoes detected negative	16	16	8	8	8	8
Unfed mosquitoes detected positive	0	0	0	0	0	0
Blood fed mosquitoes detected negative	0	3	11	14	12	14
Blood fed mosquitoes detected positive	30	27	19	16	18	16

Pv-210, Pv-247 and Pf are P. vivax 210, P. vivax 247 and P. falciparum, respectively.