Frequency distribution of the combination pvdhfr/pvdhps haplotypes obtained from 189 isolates collected in Ardebil province in north and Sistan and Baluchistan province in southeastern of Iran. The haplotype I13P33F57S58T61S117I173/A383A553 was the most prevalent among northern (84.8%) and southern (59.4%) P. vivax isolates. All six haplotypes are indicated as A to F in the figure. Mutated amino acids are boldfaced. A) I13P33F57S58T61S117I173/A383A553. B) I13P33F57S58T61N117I173/A383A553. C) I13P33F57R58T61S117I173/A383A553. D) I13P33F57R58T61N117I173/A383A553. E) I13P33L57S58T61S117I173/A383A553. F) I13P33L57R58T61S117I173/A383A553.