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Table 1 Expected spot signals for SNP positions processed by the algorithm (Results are given as a comparison with 3D7 genotype; WT = Wild type, MUT = Mutation)

From: FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance

Gene Name

SNP code

SNP name

Cy3 "green" signal

Cy5 "red" signal

Sequence

Pfdhfr

RES16

16

WT

MUT

*

Pfdhfr

RES51

51

WT

MUT

*

Pfdhfr

RES59

59

WT

MUT

*

Pfdhfr

RES108

108

MutA or MutB

WT

*

Pfdhfr

RES108B

108B

WT or MutA

MutB

*

Pfdhfr

RES164

164

WT

MUT

*

Pfdhfr

RES164B

164B

WT

MUT

*

Pfdhps

RES436

436

WT

Mut1

 

Pfdhps

RES437

437

MUT

WT

 

Pfdhps

RES540

540

WT

MUT

 

Pfdhps

RES581

581

WT

MUT

 

Pfdhps

RES613

613

MutA

WT or MutB

 

Pfdhps

RES613B

613B

WT or MutA

MutB

 

Pfdhps

RES640

640

MUT

WT

 

Pfdhps

RES645

645

MUT

WT

 

Pfmdr1

RES86

86

WT

MUT

*

Pfmdr1

RES184

184

WT

MUT

*

Pfmdr1

RES1034

1034

WT

MUT

*

Pfmdr1

RES1042

1042

MUT

WT

*

Pfmdr1

RES1246

1246

MUT

WT

*

Pfcrt

RES72

72

MUT

WT

*

Pfcrt

RES74

74

MUT

WT

*

Pfcrt

RES75

75B1

WT

MUT

*

Pfcrt

RES76

76

WT

MUT

*

Pfcrt

RES97

97

WT

MUT

*

Pfcrt

RES152

152

WT

MUT

 

Pfcrt

RES163

163

WT

MUT

 

Pfcrt

RES220

220

MUT

WT

 

Pfcrt

RES271

271

WT

MUT

 

Pfcrt

RES326

326

WT

Mut1

 

Pfcrt

RES326B

326B

Mut2

WT

 

Pfcrt

RES356

356

WT

Mut1

 

Pfcrt

RES356B

356B

WT

Mut2

 

Pfcrt

RES371

371

MUT

WT

 

PfATPase6

RES538

538

WT

MUT

*

PfATPase6

RES574

574

MUT

WT

*

PfATPase6

RES623

623

WT

MUT

*

PfATPase6

RES683

683

WT

MUT

*

PfATPase6

RES769

769

MUT

WT

*

PfATPase6

RES769B

769B

WT

MUT

*

  1. (Mutations with * were sequenced and used for our comparison with the result of the two arrays).