Figure 1

Immunoblot analyses to assess the presence of antibody against MB2 recombinant peptides. A, is a schematic representation of the MB2 protein sequence. The three domains, Basic (B), Acidic (A), and GTP-binding (G), are indicated as blocks with the amino acid junctions numbered below. The seven short horizontal lines represent the approximate extents of the polypeptides that were expressed as GST-fusion recombinant proteins. B, immunoblots of GST-MB2 recombinant proteins reacted with anti-GST rabbit serum (Anti-GST); or serum of a protected volunteer (#5 volunteer); or serum of a person living in a malaria-endemic area (Endemic serum #KU 162). Immunoblots were prepared in triplicate, and each lane contains 50-100 ng of purified GST-MB2 recombinant proteins. Recombinant proteins, MB2-C, MB2-D, and MB2-FA, listed in bold letters contain amino acid repeats. Approximate molecular weights of the fusion proteins are indicated in kilodaltons (kDa).