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Table 1 Names, positions, and sequences of oligonucleotide primers used to clone and express GST-fusion proteins representing various regions of the MB2 open reading frame.

From: Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals

GST-fusion

Amino acida

MB2-A

 

5' primer-Nco I b(98)GATGCCATGGG TGTTAATAGATGTTTTAC

 

3' primer-Sma I (305)GATCCCGGG GAGCATATTCTATTATATTCA

32 - 101

MB2-B

 

5' primer-Nco I (286)GATGCCATGG AATATAATAGAATATGCA

 

3' primer-Sma I (620)GATCCCGGG TTTTTATTATTAGAAGAATCA

95 - 206

MB2-C c

 

5' primer-Nco I (602)GATGCCATGG ATTCTTCTAATAATAAAAT

 

3' primer-Sma I (953)ATGCATCCCCGGG TCATTTTTTATTTGAAGAATTCTC

200- 316

MB2-F d

 

5' primer-Nco I (98)GATGCCATGG GTGTTAATAGATGTTTTATC

 

3' primer-Sma I (953)ATGCATCCCCGGG TCTTTTTTATTTGAAGAATTCTC

32 - 316

MB2-D

 

5' primer-Nco I (1066)GATGCCATGG CATCTACATTAGATGAAACA

 

3' primer-Sma I (1640)GATCCCGGG GATGTACTATAATCATTATTTGG

355 - 546

MB2-E

 

5' primer-Nco I (1616)GATGCCATGG ATCCAAATAATGATTATAGTACA

 

3' primer-Sma I (2321)GATCCCGGG CTTGAATTATATTCTTTATTTTCGTG

538 - 773

MB2-FA c

 

5' primer-Nco I (2294)GTATGCCATGG TCCACGAAAATAAAGAATATAATTCAAG

 

3' primer-Sma I (2837)GATCCCGGG TCATCGAGCGATTCATTTTGGTC

764 - 945

MB2-IF2

 

5' primer-Nco I (4009)GATGCCATGG ATGGTAATAGAACAAATAATGAC

 

3' primer-Sma I (4823)GATCCCGGG TACGCTTCGATTATATCGTTTGGCTC

1337 - 1606

  1. a Numbers indicate amino acid positions relative to the initiation methionine (1), b Numbers in parenthesis refer to nucleotide positions relative to the ATG initiation codon; bold underlined letters indicate the restriction endonuclease sites, Nco I and Sma I,c These peptides includes amino acid repeats, d This peptide is a combination of MB2-A, -B, and -C.