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Table 2 Optimal conditions to express GST-MB2 recombinant polypeptides in E. coli.

From: Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals

Name

Amino Acid Positiona(Size)

Net Chargeb

Mediac

Induction Time

Expression Leveld

MB2-A

32-101, (70 aa)

+8.94

SB

1 hr 10 min

1-2 mg/L

MB2-B

95-206, (112 aa)

+10.18

LB

8 hrs

3 mg/L

MB2-C

200-316, (117 aa)

+2.58

LB

4 hrs

4 mg/L

MB2-Fe(A-B-C)

32-316, (285 aa)

+21.91

SB

4 hrs

2 mg/L

MB2-D

355-546, (192 aa)

-0.72

SB

1-6 hrs

0.1-0.2 mg/L

MB2-E

538-773, (236 aa)

+8.23

SB

1-2 hrs

0.1-0.2 mg/L

MB2-FA

764-945, (182 aa)

-17.51

SB

3-6 hrs

2 mg/L

MB2-IF2

1337-1606, (270 aa)

+5.26

LB or SB

1 hr

0.5-1.0 mg/L

  1. a Position is based on the translation initiation methionine (1).
  2. b Calculated at neutral pH
  3. c LB, Luria Broth; SB, Super Broth. Media were made according to instructions in Sambrook et al.,[39].
  4. d Expressed as amount of purified protein per volume of IPTG-induced culture
  5. e Combined length of MB2-A+MB-2B+MB2-C