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Figure 1 | Malaria Journal

Figure 1

From: Plasmodium falciparum exposure in utero, maternal age and parity influence the innate activation of foetal antigen presenting cells

Figure 1

(A) Flow cytometric identification of MDC (BDCA-1+ or BDCA-3+) and PDC (BDCA-2+) in CBMC from one subject. R1 gate was set to include only viable mononuclear cells, as determined by forward scatter (FSC) and side-scatter (SSC) characteristics. Furthermore, cells were gated to be both CD14 and CD19 negative (R2) and either BDCA-1, BDCA-3, or BDCA-2 positive (R3). One million events were analysed for DC, 300,000 events for monocytes and 100,000 events for isotype control enumeration. The percentages represent relative levels of the different DC populations from the selected subject. (B) Partial activation of cord blood BDCA-1+ and BDCA-2+ cells is related to the presence of MP in placenta. The expression levels of HLA-DR and CD86 were measured by flow cytometry on foetal APC subsets from 16 MP-positive (diagonal striped bars) and 39 MP-negative mothers (white bars). Boxplots illustrate the medians and the 25th and 75th percentiles. Y-axes report percentage of positive cells and mean fluorescence intensity (MFI) for the activation markers CD86 and HLA-DR in different APC subsets. P-values were calculated by Mann-Whitney U test. The significance limit was P < 0.05.

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