Plasmodium falciparum exposure in utero, maternal age and parity influence the innate activation of foetal antigen presenting cells

Table 2 APC absolute numbers and immunophenotype in cord blood samples.

	APC subset
Parameter: median (± interquartile)	Monocytes	MDC		PDC
	CD14 ⁺	BDCA-1 ⁺	BDCA-3 ⁺	BDCA-2 ⁺
% of total CBMC	13.00 ± (7.10)	0.62 ± (0.42)	0.19 ± (0.18)	0.25 ± (0.18)
absolute no./ml	624,787 ± (571,630)	23,850 ± (30,430)	8,160 ± (12,225)	9,680 ± (9,032)
% HLA-DR⁺ cells	93.87 ± (7.09)	93.53 ± (11.01)	59.95 ± (28.55)	82.64 ± (31.69)
HLA-DR, MFI^a	132.71 ± (17.69)	162.50 ± (18.81)	118.26 ± (40.81)	129.9 ± (39.59)
% CD86⁺ cells	50.28 ± (34.37)	44.67 ± (22.15)	35.95 ± (20.98)	18.47 ± (13.41)
CD86, MFI	62.36 ± (30.17)	57.51 ± (21.12)	45.54 ± (17.80)	33.29 ± (13.77)

Immunophenotyping was performed on 55 cord blood samples; 27 from malaria infected women and 28 from uninfected women. APC were quantified as a percentage of the total CBMC. Absolute numbers of MDC, PDC and monocytes were calculated from CBMC counts. HLA-DR and CD86 expression levels were then examined in different APC subsets.
^a; MFI, mean fluorescence intensity

ISSN: 1475-2875