Figure 1

Identification of members of the An. funestus group by melt-curve real-time PCR analysis. In this example five to seven specimens of An. rivulorum (red trace), An. leesoni (blue trace), An. funestus (brown trace), An. parensis (green trace) and An. vaneedeni (pink trace) were tested. A plot of negative first derivative of the collected fluorescence against temperature results in melt peaks with characteristic melting temperatures (T_M).