Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group

Table 1 Primers and probes used in the identification of Anopheles funestus species using real time assays (MCA, HRM and TaqMan)

Name	Primer /probe	Reporter Dye	Sequence (5'-3')	Quencher
FUN1	Primer	-	GGCATCGATGGGTTAATCATG	-
VAN1	Primer	-	AAACCCCAAGATGTGCTCC	-
VAN3	primer	-	GGTTTTCAAATGAATCTC	-
N PAR	Primer	-	ATACTTGTGTGTGTGTGTATTTG	-
RIV TM	probe	Cy5	CTATGGCGAGACCCCGTCTAGTG	BHQ2^a
FUN TM	probe	ROX	CATGGGGAAATTCAATCGAAAACCTCT	BHQ2^a
PAR TM	probe	VIC	CGG AAC CTA GCT TGG	MGBNFQ^b
VAN TM	probe	Quasar 705	CGT TGT GAA AAA TGG AGA TTC ATT TGA AAA CC	BHQ2^a
LEES TM	probe	6-FAM	CCG ACC GAT GTA CA	MGBNFQ^b

ISSN: 1475-2875