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Table 5 Average stage-specific detection limit of clinical specimens (n = 3 per species) by QPCR.

From: Multiplex real-time quantitative PCR, microscopy and rapid diagnostic immuno-chromatographic tests for the detection of Plasmodium spp: performance, limit of detection analysis and quality assurance

  Parasite stage specific limit of detection
rDNA copy number per reaction (parasites per reaction)
Single Rings Double Rings Growing Trophozoites Mature Trophozoites Gametocytes Mature Schizonts Immature Schizonts
P. falciparum 6.21 ± 2.0
(1.08 ± 0.34)
1.74 ± 1.09 (0.29 ± 0.18)    30
(5)
  
P. vivax 3.66 ± 2.3
(0.60 ± 0.4)
  4.51 ± 0.4
(0.75 ± 0.07)
21.72
(3.6)
0.590
(0.098)
  
P. malariae 5.90 ± 4.29 (0.95 ± 0.75)   4.74 ± 2.490 (0.78 ± 0.415) 82.2
(13.7)
3.03 ± 1.7
(0.5 ± 0.3)
4.475 ± 1.9
(0.705 ± 0.2)
4.15 ± 0.94 (0.69 ± 0.16)
P. ovale 0.56 ± 0.18 (0.08 ± 0.02)   18 ± 4.5
(3 ± 0.75)
  0.18
(0.03)
6
(1)
 
  1. LOD are stated as either ribosomal DNA copy number or parasites per reaction with standard error where multiple stages were present in specimens.