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Figure 4 | Malaria Journal

Figure 4

From: Chloroquine reduces arylsulphatase B activity and increases chondroitin-4-sulphate: implications for mechanisms of action and resistance

Figure 4

Extracted ion chromatography (EIC) representation of disaccharide composition in cerebrovascular cells before and after exposure to chloroquine. The HPLC system used resolves the eight CS disaccharide standards (Panel A). Despite the variability of retention time due to variable quantities of residual buffer salts, peak identity was easily confirmed by co-injection with single known CS disaccharide standards as well as from their MS (see Additional File 1) or by MS/MS spectra. CVC controls (Panels B, D, and F) and CVC after chloroquine treatment (Panels C, E, and G) are shown. The chloroquine-treated CVC demonstrate marked increase in the peak for C4S disaccharide (ΔDi-4S) following chloroquine exposure (Panels E and G vs. Panel F). Additional peaks observed early (3-8 min) in the chromatograms B, D, and F and well separated from the sulpho-group containing CS standards are residual contaminants from cells and media and do not interfere with the analysis.

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Malaria Journal

ISSN: 1475-2875

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