Influence of adjuvant on anti-MSP4 and MSP5 IgG production. The adjuvant effect of AFCo1, SCS or CFA on the immune response against MSP4 or MSP5 was explored in separated immunization experiments. Mice were injected with three intramuscular doses at a 14-day interval of MSP4 or MSP5 incorporated into AFCo1 or SCS, or mixed with CFA or PBS. Serum samples were taken 21 days after the last immunization, MSP4 (Panel A) or MSP5 (Panel B)-specific IgG were measured by ELISA. Antibodies levels are expressed as a logarithm of the titer. Each experiment was repeated 3 times. The means and standard deviations are showed. * Significant differences by Tukey test (p < 0.05) compared with PBS group.