Influence of adjuvant on anti-MSP4 and MSP5 IgG isotype production. The adjuvant effect of AFCo1, SCS, CFA or PBS on the immune response against MSP4 or MSP5 was compared. Mice were injected with three intramuscular doses at a 14 day interval of MSP4 or MSP5 incorporated into AFCo1 or SCS, or mixed with CFA or PBS. MSP-specific IgG1 and IgG2a were measured by ELISA in serum taken 21 days after the last immunization. (Panel A) MSP4-specific IgG1 (open bars) and IgG2a (lined bars) from MSP4 immunized mice. (Panel B) MSP5-specific IgG1 (filled bars) and IgG2a (lined bars) from MSP5 immunized mice. Antibodies levels are expressed as a logarithm of the titer. The means and standard deviations of 3 different experiments are shown. * Significant differences by Tukey test (p < 0.05).).