Delayed type hypersensitivity reaction induced by intradermal challenge with MSP4 or MSP5 in immunized mice. Mice were immunized with MSP4 or MSP5 incorporated into AFCo1 or SCS, or mixed with CFA or PBS (3 intramuscular doses, 14 day interval). The presence of delayed type hypersensitivity (DTH) was tested 21 days after the last immunization by intradermal challenge with 5 μg of MSP4 or MSP5 in the left footpad. PBS was injected in the right footpad as control. The size of inflammation in both footpaths was measured 48 h after challenge using a caliper. The induration induced by the antigen was measured as the difference of right minus left footpad thickness. The magnitude of DTH reaction induced by MSP4 (Panel A) and MSP5 (Panel B) immunization are expressed as the mean and standard deviation of the induration in each experimental group. * Significant differences by Tukey test (p < 0.05).