Figure 1

Digoxigenin(DIG)-labeled heteroduplex tracking assay. Panel A shows a dilution series of known mixtures of PCR amplified genomic DNA detected by chemiluminescence and autoradiography. Lanes A to D represent controls (water, NTC, Wild type DNA, and Mutant DNA). Mixtures of 50%, 20%, 10%, 5%, 1% and 0.1% mutant DNA are seen in lanes E to J, repectively. Panel B shows a similar dilution series detected by chemiluminesence using the LAS-3000. Panel C shows clinical samples tested by chemiluminescence with autoradiography. Lanes A to D are controls in the same order as Panel A. Lanes E, F, K and L contain samples in duplicate that are pure wild type DNA. Lanes G, H, I and J contain samples in duplicate of mixed parasitaemias. The lowest band on all the gels represents the probe homoduplex.