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Table 1 Quantification of band intensity by the Digoxigenin(DIG)-labeled heteroduplex tracking assay

From: Nonradioactive heteroduplex tracking assay for the detection of minority-variant chloroquine-resistant Plasmodium falciparum in Madagascar

% Mutant DNA

Autoradiography1

(ImageJ)

 

Chemiluminescent

(Multi Gauge)

 

Chemifluorescent

(ImageQuant)

 

Average %

SD

Average %

SD

Average %

SD

50%

45.4

2.7

44.3

1.6

43.5

8.7

20%

29.2

2.5

26.0

2.2

16.2

5.0

10%

15.6

7.5

17.5

5.8

8.7

2.4

5%

8.1

2.2

9.0

3.8

5.4

2.2

1%

1.72

2.2

2.9

1.5

0.852

0.72

0.1%

ND3

ND

ND

ND

ND

ND

  1. 1: Chemiluminescent detection.
  2. 2: Detected in 3 of 4 replicates.
  3. 3: Not detected