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Table 1 Quantification of band intensity by the Digoxigenin(DIG)-labeled heteroduplex tracking assay

From: Nonradioactive heteroduplex tracking assay for the detection of minority-variant chloroquine-resistant Plasmodium falciparum in Madagascar

% Mutant DNA Autoradiography1 (ImageJ)   Chemiluminescent (Multi Gauge)   Chemifluorescent (ImageQuant)  
Average % SD Average % SD Average % SD
50% 45.4 2.7 44.3 1.6 43.5 8.7
20% 29.2 2.5 26.0 2.2 16.2 5.0
10% 15.6 7.5 17.5 5.8 8.7 2.4
5% 8.1 2.2 9.0 3.8 5.4 2.2
1% 1.72 2.2 2.9 1.5 0.852 0.72
0.1% ND3 ND ND ND ND ND
  1. 1: Chemiluminescent detection.
  2. 2: Detected in 3 of 4 replicates.
  3. 3: Not detected