Figure 2

GIMAP1 is expressed endogenously in various immune cell populations and in cell lines. Cells were purified from C57BL/6 mice by FACS sorting with markers as follows: T cells (CD3+), B cells (B220+), non T/B cells (CD3- B220-), DN thymocytes (CD4- CD8-), DP thymocytes (CD4+ CD8+), CD4+ SP thymocytes (CD4+ CD8-), CD8+ SP thymocytes (CD4- CD8+), Pro B/Pre BI cells (B220+ CD25- IgD- IgM-), PreBII cells (B220+ CD25+ IgD- IgM-), immature B cells (B220+ CD25- IgD- IgM+), mature B cells (B220+ CD25- IgD+ IgM+), NK cells (NK1.1+), macrophages (F4/80+). Cell lysates were Western blotted with MAC 420 anti-GIMAP1 or anti-β actin. B. Lysates from the indicated mouse lymphomyeloid cell lines were Western blotted with MAC 420 anti-GIMAP1, a rabbit anti-GIMAP1 polyclonal antibody or anti-β actin.