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Figure 3 | Malaria Journal

Figure 3

From: Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria

Figure 3

GIMAP1 is not up-regulated in mice infected with Plasmodium chabaudi AS or CB. C57BL/6 and C57BL/10 mice were infected with Plasmodium chabaudi AS or CB (a more virulent strain of the parasite). Seven days post-infection mice were culled and the levels of GIMAP1 protein and mRNA were measured. A. GIMAP1 protein levels were measured by Western blotting of spleen cell lysates from naïve or infected mice using the GIMAP1 monoclonal antibody (MAC420). Anti-β actin served as a loading control. B. The intensities of GIMAP1 bands in the Western blot in A were quantified by densitometry and plotted relative to the average intensity of the bands for the C57BL/6 naïve mice. C. and D. RNA was extracted from the spleen of each mouse and Real-time PCR was used to measure the levels of GIMAP1 relative to the average of two naïve mice, which was set to a value of 1. Bars represent individual mice.

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