Figure 4

GIMAP1 is not up-regulated in C57BL/10 mice immune to Plasmodium chabaudi infection. C57BL/10 mice were immunised with erythrocyte ghosts from infected mice in Freund's adjuvant. Seven days later the mice were infected with Plasmodium chabaudi AS. After nine weeks the mice were infected a second time. Seven days post-infection the mice were culled and analysed for GIMAP1 expression (immune and infected mice). A. Spleen lysates were Western blotted with the anti-GIMAP1 mAb MAC420 or anti-β actin. B. RNA was extracted from spleens, cDNA was synthesized and real-time PCR was used to measure relative GIMAP1 levels. 'PBS' denotes mice that were given PBS alone instead of erythrocyte ghosts and were not infected; 'Freund's' denotes mice that were given Freund's adjuvant without erythrocyte ghosts and were not infected. Levels of GIMAP1 were measured relative to the levels of two control genes, cirhin and 6-phosphofructokinase.