Figure 5
GIMAP1 was not up-regulated in mice immune to malaria infection or in mice which had received other combinations of the treatments to make the mice immune. A. Details of the treatment of each group of mice: mice were immunised with erythrocyte ghosts from naïve or parasitised mice in Freund's adjuvant, or were given Freund's alone, or PBS. Seven days later mice were infected with 106 Plasmodium chabaudi AS parasites (Infection 1, Y), or were given Krebs' saline alone (N). After nine weeks mice were infected with 106 Plasmodium chabaudi AS parasites (Infection 2, Y), or were given Krebs' saline alone (N). On day 7 post infection mice were culled and analysed for GIMAP1 expression. B. Representative spleen lysates were separated by SDS-PAGE and Western blotted with the anti-GIMAP1 mAb MAC420 and anti-β-actin. C. The expression of GIMAP1 mRNA in spleen samples was measured by real-time PCR, arbitrarily setting the mean of the two Group A samples at 1. Mean values for GIMAP1 expression levels for each group of mice are shown in the lower histogram. Mouse group designations are given beneath the histograms.