Figure 6

Relative expression of GIMAP genes in bone marrow B cell subpopulations from C57BL/6 mice. Real-time PCR was used to compare GIMAP gene expression in bone marrow B cell subpopulations with that in lymph node B cells, lymph node CD4+ T cells and DN thymocytes. (T cells [black square]; B cells: [grey square]). Bar 1: DN (CD4-CD8-) thymocytes (reference); bar 2: lymph node CD4+CD8- T cells (control, strong expression); bar 3: bone marrow pro-B and pre-BI; bar 4: bone marrow pre-BII; bar 5: bone marrow immature B; bar 6: bone marrow mature B cells and bar 7: LN CD19+ B cells. (A-H) Expression levels of, respectively, GIMAP1, GIMAP3, GIMAP4, GIMAP5, GIMAP6, GIMAP7, GIMAP8 and GIMAP9. ↔ Reference level (set at 1) corresponding to the DN population. The data are representative of three separate experiments conducted in triplicate. Cell populations were purified by FACS using the cell surface markers indicated (or see Fig.2 legend for cell surface markers for bone marrow B cell subsets).